Chromatin and Cytoskeletal Tethering Determine Nuclear Morphology in Progerin Expressing Cells

The nuclear morphology of eukaryotic cells is determined by the interplay between lamina forming skeleton, chromatin inside nucleus, and coupling with cytoskeleton. Nuclear alterations are often associated pathological conditions as in Hutchinson-Gilford progeria syndrome (HGPS), which a mutation lamin A gene yields an altered form protein, named progerin, aberrant shape. While genetic molecular basis disease have been widely elucidated, biophysical effect progerin expression on functioning still unsolved issue. In present talk, I will discuss our recent work we introduce inducible cellular model HGPS HeLa where increased leads to significant modification similar those observed patient's derived cells, organization. Moreover, results show that induction reduced interactions lamins tethers well polycomb group proteins and, at same time, enhanced cytoskeleton-nuclear shell tethering resulting shape fluctuations response cytoskeletal activity. Overall, data highlights how affects, crucial ways, cytoskeleton, lamins, epigenetic regulation status.